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- Title
Family of the major cold-shock protein, CspA (CS7.4), of Escherlchia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins.
- Authors
Sang Jun Lee; Aiguo Xie; Weining Jiang; Etchegaray, Jean-Pierre; Inouye, Masayori; Jones, Pamela G.
- Abstract
The <em>cspA</em> is a gene of <em>Escherichia coli</em>, whose expression is specifically induced at low temperatures to a level of 13% of total protein synthesis. The CspA protein consisting of 70 amino acid residues has high sequence similarity with eukaryotic Y-box DNA-binding proteins. We found two independent clones from the Kohara miniset phage collection, which hybridized with a DNA fragment containing <em>cspA</em>. DNA sequencing of these clones confirmed that the two genes are highly homologous to <em>cspA </em>. One designated <em>cspB</em> is mapped at 35 min on the <em>E. coli</em> chromosome and encodes a 71-residue protein with 79% identity to CspA, while the other, <em>cspC</em>, is mapped at 40 min and encodes a 69-residue protein with 70% identity, in addition, a DNA sequence upstream of the <em>clpA</em> gene at 19 min published elsewhere contains an open reading frame for a 74-residue protein with 45% identity to CspA. All <em>csp</em> genes were fused in the coding regions with the <em>lacZ</em>gene, and the expression of β-galactosidase was examined for these hybrid genes upon cold shock. A similar cold-shock induction to <em>cspA</em> was observed for <em>cspB</em> but not <em>cspC</em> and <em>cspD</em>. These results indicate that <em>E. coli</em> has a family of the <em>cspA</em> gene, some of which are induced by cold shock.
- Subjects
GENES; ESCHERICHIA coli; GENE expression; LOW temperatures; AMINO acids
- Publication
Molecular Microbiology, 1994, Vol 11, Issue 4, p833
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1994.tb00361.x