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- Title
Characterization of hydrogen sulphide reaction with rat-tail tendon Type I collagen <em>in vitro</em>.
- Authors
Johnson, P. W.; Tonzetich, J.; Pearce, R. H.
- Abstract
Type I acid-soluble rat-tail tendon collagen was reacted up to four days with hydrogen sulphide (H2S) concentration equivalent to that produced by putrefying saliva in <em>in vitro</em> systems. After reaction, the head-space was assayed for H2S by gas chromatography (GC), and the reaction mixture was separated into salt-soluble supernatant fraction and sediment which was solubilized in 0.5 M acetic acid. Both fractions were analyzed by SDS-polyacrylamide gel disc electrophoresis (SDS-PAGE) and assayed for free aldehyde, free thiol, total available thiol, and hydroxyproline content. The GC analysis of head-space samples showed that more than 85% of the added H2S was absorbed within the initial 24 h by the reaction medium and totally removed from the head-space in four days. Although no discernible qualitative or quantitative changes were visually detected in H2S-treated systems by SDS-PAGE, increased levels of hydroxyproline in the supernatant fraction indicated production of a neutral salt-soluble product. Chemical analysis showed that the reaction resulted in the incorporation of H2S in free thiol and disulphide forms in both fractions. [35S]-H2S/paper chromatography methods confirmed that H2S was incorporated into collagen. An increase in free aldehyde groups of both fractions inferred exposure of aldehyde groups presumably through cleavage of intramolecular cross-linkages. The reversion of acid-soluble collagen to a more soluble product. which is considered more susceptible to enzymatic degradation. suggests one mechanism how the volatile thiol compounds may contribute to the etiology of periodontal disease.
- Subjects
ETIOLOGY of diseases; COLLAGEN; PERIODONTAL disease; SALIVARY glands; COLLOIDS; CHROMATOGRAPHIC analysis
- Publication
Journal of Periodontal Research, 1985, Vol 20, Issue 4, p403
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.1985.tb00452.x