We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Cajaninstilbene Acid Relaxes Rat Renal Arteries: Roles of Ca<sup>2+</sup> Antagonism and Protein Kinase C-Dependent Mechanism.
- Authors
Dong-Mei Zhang; Yong Li; Wai San Cheang; Chi Wai Lau; Shun-Ming Lin; Qian-Lan Zhang; Nan Yao; Ying Wang; Xin Wu; Yu Huang; Wen-Cai Ye
- Abstract
Cajaninstilbene acid (CSA) is a major active component present in the leaves of Cajanus cajan (L.) Millsp. The present study explores the underlying cellular mechanisms for CSA-induced relaxation in rat renal arteries. Vascular reactivity was examined in arterial rings that were suspended in a Multi Myograph System and the expression of signaling proteins was assessed by Western blotting method. CSA (0.1-10 µM) produced relaxations in rings pre-contracted by phenylephrine, serotonin, 9, 11-dideoxy-9α, 11α-epoxymethanoprostaglandin F2α (U46619), and 60 mM KCl. CSA-induced relaxations did not show difference between genders and were unaffected by endothelium denudation, nor by treatment with NG-nitro-Larginine methyl ester, indomethacin, ICI-182780, tetraethylammonium ion, BaCl2, glibenclamide, 4-aminopyridine or propranolol. CSA reduced contraction induced by CaCl2 (0.01-5 mM) in 2+-free 60 mM KCl solution and by 30 nM (--)-Bay K8644 in 15 mM KCl solution. CSA inhibited 60 mM KCl-induced 2+ influx in smooth muscle of renal arteries. In addition, CSA inhibited contraction evoked by phorbol 12-myristate 13-acetate (PMA, protein kinase C agonist) in 2+-free Krebs solution. Moreover, CSA reduced the U46619- and PMA-induced phosphorylation of myosin light chain (MLC) at Ser19 and myosin phosphatase target subunit 1 (MYPT1) at Thr853 which was associated with vasoconstriction. CSA also lowered the phosphorylation of protein kinase C (PKCδ) at Thr505. In summary, the present results suggest that CSA relaxes renal arteries in vitro via multiple cellular mechanisms involving partial inhibition of calcium entry via nifedipine-sensitive calcium channels, protein kinase C and Rho kinase.
- Subjects
PIGEON pea; RENAL artery; PROTEIN research; WESTERN immunoblotting; PHENYLEPHRINE; SEROTONIN; PROTEIN kinase C
- Publication
PLoS ONE, 2012, Vol 7, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0047030