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- Title
An Electron Microscopic India Ink Technique for Demonstrating Capsules on Microorganisms: Studies with Streptococcus pneumoniae, Staphylococcus aureus, and Neisseria gonorrhoeae.
- Authors
Melly, M. Ann; McGee, Zell A.; Horn, Robert G.; Morris, Fred; Glick, Alan D.
- Abstract
A technique using India ink in electron microscopic preparations was used to study bacterial capsules. Capsules were demonstrated on Streptococcus pneumoniae and Staphylococcus aureus (strain M and the Smith diffuse variant) from in vitro cultures. Two types of false capsules were observed on Neisseria gonorrhoeae grown in vitro or obtained from human urethral exudates. No true capsules were definitively identified on gonococci from these sources. The technique described does not require the use of specific anticapsular antibody or specific polysaccharide stains and permits the detection of artifacts that may, by the use of light microscopicmethods, be misinterpreted as capsules.
- Publication
Journal of Infectious Diseases, 1979, Vol 140, Issue 4, p605
- ISSN
0022-1899
- Publication type
Article