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- Title
Recent developments in protein-ligand affinity mass spectrometry.
- Authors
Jonker, Niels; Kool, Jeroen; Irth, Hubertus; Niessen, Wilfried M. A.
- Abstract
This review provides an overview of direct and indirect technologies to screen protein-ligand interactions with mass spectrometry. These technologies have as a key feature the selection or affinity purification of ligands in mixtures prior to detection. Specific fields of interest for these technologies are metabolic profiling of bioactive metabolites, natural extract screening, and the screening of libraries for bioactives, such as parallel synthesis libraries and small combichem libraries. The review addresses the principles of each of the methods discussed, with a focus on developments in recent years, and the applicability of the methods to lead generation and development in drug discovery. [Figure not available: see fulltext.]
- Subjects
PROTEIN affinity labeling; LIGANDS (Chemistry); MASS spectrometry; BIOLOGICAL assay; METABOLITES; BIOACTIVE compounds
- Publication
Analytical & Bioanalytical Chemistry, 2011, Vol 399, Issue 8, p2669
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-010-4350-z