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- Title
Disruption of non-anchored cell wall protein NCW-1 promotes cellulase production by increasing cellobiose uptake in Neurospora crassa.
- Authors
Lin, Liangcai; Chen, Yong; Li, Jingen; Wang, Shanshan; Sun, Wenliang; Tian, Chaoguang
- Abstract
Objectives: To elucidate the mechanism of cellulase signal transduction in filamentous fungi including the components of the cellulase induction pathway. Results: Neurospora crassa ncw- 1 encodes a non-anchored cell wall protein. The absence of ncw- 1 increased cellulase gene expression and this is not due to relieving carbon catabolite repression mediated by the cre- 1 pathway. A mutant lacking genes encoding both three major β-glucosidase enzymes and NCW-1 (Δ3βGΔ ncw- 1) was constructed. Transcriptome analysis of the quadruple mutant demonstrated enhanced expression of cellodextrin transporters after ncw- 1 deletion, indicating that ncw- 1 affects cellulase expression and production by inhibiting the uptake of the cellodextrin. Conclusions: NCW-1 is a novel component that plays a critical role in the cellulase induction signaling pathway.
- Subjects
BACTERIAL cell walls; CELLULASE regulation; NEUROSPORA crassa; CELLOBIOSE; CELLULAR signal transduction; GENE expression
- Publication
Biotechnology Letters, 2017, Vol 39, Issue 4, p545
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-016-2274-1