We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model.
- Authors
Yazdani, Yaghoub; Keyhanvar, Neda; Kalhor, Hamid; Rezaei, Abbas
- Abstract
Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation ( p < 0.01).
- Subjects
FUNCTIONAL analysis; HEPCIDIN; RECOMBINANT proteins; CELL culture; ANIMAL models in research; PEPTIDE hormones
- Publication
Biotechnology Letters, 2013, Vol 35, Issue 8, p1191
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1198-2