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- Title
An improved method for Beauveria bassiana transformation using phosphinothricin acetlytransferase and green fluorescent protein fusion gene as a selectable and visible marker.
- Authors
Kai Jin; Yongjun Zhang; Zhibing Luo; Yuehua Xiao; Yanhua Fan; Di Wu; Yan Pei
- Abstract
An improved transformation method for the biocontrol agent, Beauveria bassiana, was developed. For convenience of transformation selection and detection, the coding regions of the genes for phosphinothricin acetlytransferase and green fluorescent protein were fused and an expression vector, pBFT, carrying this fusion was constructed. Under optimum conditions, over 60 transformants μg−1 plasmid DNA were obtained. B. bassiana conidia frozen 1 month at −80°C were fully competent for transformation. The method was significantly less laborious and more rapid than current methods for B. bassiana. The bar::egfp provides a selectable and visible marker which may expedite future genetic engineering of this fungus.
- Subjects
GREEN fluorescent protein; BIOMARKERS; FLUORESCENT polymers; NUCLEIC acids; GENETIC recombination; GENETIC engineering; GENETIC vectors; GENE expression; BIOTECHNOLOGY
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 8, p1379
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9713-6