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- Title
Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection.
- Authors
Ferrari, B. C.; Power, M. L.; Bergquist, P. L.
- Abstract
Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.
- Subjects
NUCLEIC acids; PROTEOLYTIC enzymes; DNA; PROTEINASES; PARASITES; NEMATODES; APICOMPLEXA; WORMS; GENES
- Publication
Biotechnology Letters, 2007, Vol 29, Issue 12, p1831
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-007-9487-2