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- Title
Optimization of an effective directed differentiation medium for differentiating mouse bone marrow mesenchymal stem cells into hepatocytes in vitro
- Authors
Shi, Xiao-Lei; Mao, Liang; Xu, Bi-Yun; Xie, Ting; Zhu, Zhang-Hua; Chen, Jun-Hao; Li, Lei; Ding, Yi-Tao
- Abstract
Abstract: We have used a uniform design to explore the most effective directed differentiation medium (MEDDM) for differentiating mouse bone marrow mesenchymal stem cells (mMSCs) into hepatocytes. Our methods involved arranging eight differentiation medium groups following uniform design. Flow cytometry was used to evaluate the percentage of ALB+ and CK18+ cells in each group. Factors and their concentrations in the MEDDMs were then identified. The MEDDMs were evaluated by their ability to differentiate mMSCs into hepatocytes by RNA and protein expressions and synthesis functions. FGF at 35ng/ml and OSM at 30ng/ml in the medium yielded the highest percentage of ALB+ and CK18+ cells. During directed differentiation using MEDDMs, ALB, CK18, TTR, AFP mRNAs were expressed. ALB and CK18 proteins were detected in the cells. The differentiated cells produced albumin and urea in a time dependent manner. Uniform design was adequate for choosing the MEDDM of mMSCs. MEDDM containing 35ng/ml FGF and 30ng/ml OSM was effective in differentiating mMSCs into hepatocytes.
- Subjects
MESENCHYME; BONE marrow; LIVER cells; NUCLEIC acids
- Publication
Cell Biology International, 2008, Vol 32, Issue 8, p959
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2008.04.013