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- Title
Evaluation of PCR-based Quantification Techniques to Estimate the Abundance of Atrazine Chlorohydrolase Gene atzA in Rhizosphere Soils.
- Authors
Thompson, Brian M.; Chung-Ho Lin; Hsin-Yeh Hsieh; Kremer, Robert J.; Lerch, Robert N.; Garrett, Harold E.
- Abstract
The article presents a study on polymerase chain reaction (PCR) based quantification techniques to estimate the abundance of atrazine chlorohydrolase gene atzA in rhizosphere soils. The study compares four quantitative methods for enumeration of atrazine-degrading bacteria in rhizosphere environments and utilizes the optimal probe-based real-time PCR-based method in an ongoing intermediation experiment to monitor atzA copy number over time. The goal of the study is to quantify atzA copy number in rhizosphere soil samples using four distinct quantitative methods and apply the most accurate and reliable quantitative method for quantification of atzA in comparison to degradation kinetics in rhizosphere environment.
- Subjects
POLYMERASE chain reaction; PREDICATE calculus; SOIL testing; SOIL quality; PHYSIOLOGICAL effects of atrazine; RHIZOSPHERE; RHIZOBACTERIA; BIOREMEDIATION; BIOTECHNOLOGY research
- Publication
Journal of Environmental Quality, 2010, Vol 39, Issue 6, p1999
- ISSN
0047-2425
- Publication type
Article
- DOI
10.2134/jeq2010.0192