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- Title
Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1.
- Authors
Grimm, Clemens; Pelz, Jann-Patrick; Schneider, Cornelius; Schäffler, Katrin; Fischer, Utz
- Abstract
Eukaryotic cells determine the protein output of their genetic program by regulating mRNA transcription, localization, translation and turnover rates. This regulation is accomplished by an ensemble of RNA-binding proteins (RBPs) that bind to any given mRNA, thus forming mRNPs. Poly(A) binding proteins (PABPs) are prominent members of virtually all mRNPs that possess poly(A) tails. They serve as multifunctional scaffolds, allowing the recruitment of diverse factors containing a poly(A)-interacting motif (PAM) into mRNPs. We present the crystal structure of the variant PAM motif (termed PAM2w) in the N-terminal part of the positive translation factor LARP4B, which binds to the MLLE domain of the poly(A) binding protein C1 cytoplasmic 1 (PABPC1). The structural analysis, along with mutational studies in vitro and in vivo, uncovered a new mode of interaction between PAM2 motifs and MLLE domains.
- Subjects
CRYSTAL structure; RNA-binding proteins; CARRIER proteins; EUKARYOTIC cells; IN vitro studies
- Publication
Biomolecules (2218-273X), 2020, Vol 10, Issue 6, p872
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom10060872