We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model.
- Authors
Ashley, Richard A.; Roth, Christopher C.; Palmer, Blake W.; Kibar, Yusuf; Routh, Jonathan C.; Fung, Kar-Ming; Frimberger, Dominic; Hsueh-Kung Lin; Kropp, Bradley P.
- Abstract
OBJECTIVE To examine the histological differences in the inflammatory response and regenerative outcomes of distal vs proximal porcine small intestinal submucosa (SIS) grafts in the rat bladder, as SIS from distal small intestine yields reliable and reproducible bladder regeneration, while SIS from proximal portions of small intestine does not provide similar results. MATERIALS AND METHODS In all, 30 Sprague-Dawley rats underwent hemi-cystectomy followed by anastomosis of a bladder patch of SIS prepared from either distal or proximal small intestine. After bladder harvest, immunohistochemistry was used to quantify mast cells, eosinophils, macrophages, and neutrophils (PMNs). Total cell count per unit area was compared across the time course in univariate and logistic regression modelling. RESULTS There were more eosinophils and mast cells in proximal SIS grafts, while there were more macrophages and PMNs in distal SIS grafts (all P < 0.05). Trichrome analysis showed increased collagen deposition in proximal SIS grafts and little smooth muscle regeneration. There was also significant graft contracture in proximal SIS grafts compared with distal SIS grafts ( P < 0.05). CONCLUSIONS We conclude that the location of SIS origin may evoke different inflammatory responses, which results in altered bladder tissue regeneration.
- Subjects
INFLAMMATION; REGENERATION (Biology); SMALL intestine; SPRAGUE Dawley rats; BLADDER; SURGICAL anastomosis; IMMUNOHISTOCHEMISTRY
- Publication
BJU International, 2010, Vol 105, Issue 10, p1462
- ISSN
1464-4096
- Publication type
Article
- DOI
10.1111/j.1464-410X.2009.08965.x