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- Title
Cytochrome Oxidase 2 (COX2), β-Tubulin (TUB) and Chitin Synthase Subunit 2 (CHS2) Expression in Pythium insidiosum Thai Strains.
- Authors
KAMMARNJASSADAKUL, Patcharee; RANGSIPANURATN, Watcharin; SRIPRAPUN, Methee; NGAMSAKULRUNGRUJ, Popchai; PALAGA, Tanapat; SRITUNYALUCKSANA, Kallaya; CHINDAMPORN, Ariya
- Abstract
This study aimed to investigate the real-time fluctuation of temperature-sensitive gene expression in a Pythium insidiosum growing at human body temperature (37 °C) when it can cause life-threatening disease, whereas its natural habitat is in agricultural water sources with lower temperature. Genes encoding cytochrome oxidase 2 (COX2), ß-tubulin (TUB), and chitin synthase subunit 2 (CHS2) were tested for their expression at the infection temperature (37 °C) and natural habitat temperature (27 °C) using real-time RT-PCR. Sixteen strains of P. insidiosum, representing 3 phylogeographic preferences, were analyzed. The subculturing process at 27 °C was repeated at least 2 - 3 cycles. The results showed that at 37 °C, the COX2 was expressed 2.5-fold higher than that of 27 °C (p = 0.0347) while the level of TUB and CHS2 mRNA disclosed indistinguishable profiles. These results suggested that these genes were suitable to be used as housekeeping genes for temperature-susceptible gene expression studies. This work was likely to be the 1st study examining the gene expression levels of the Thai strain of P. insidiosum under thermal stress conditions. Follow-up studies for the COX2 genes may useful to provide valuable insight into the pathogenesis, diagnostic, or therapeutic targets for further investigation.
- Subjects
CHITIN synthase; PYTHIUM; GENES; THERMAL stresses; GENE expression; CYTOCHROME oxidase; TUBULINS
- Publication
Walailak Journal of Science & Technology, 2021, Vol 18, Issue 8, p1
- ISSN
1686-3933
- Publication type
Article
- DOI
10.48048/wjst.2021.9433