We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A noninvasive, direct real-time PCR method for sex determination in multiple avian species.
- Authors
BRUBAKER, JESSICA L.; KAROUNA-RENIER, NATALIE K.; YU CHEN; JENKO, KATHRYN; SPRAGUE, DANIEL T.; HENRY, PAULA F. P.
- Abstract
Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.
- Subjects
POLYMERASE chain reaction; BIRDS; SEXING of animals; ANIMAL species; GEL electrophoresis
- Publication
Molecular Ecology Resources, 2011, Vol 11, Issue 2, p415
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/j.1755-0998.2010.02951.x