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- Title
Influence of different metals on the activation and inhibition of α-amylase from thermophilic Bacillus firmus KIBGE-IB28.
- Authors
Zohra, Raheela Rahmat; Ul Qader, Shah Ali; Pervez, Sidra; Aman, Afsheen
- Abstract
Thermophilic Bacillus firmus KIBGE-IB28 produced extracellular a-amylase at temperature 70°C. Enzyme was partially purified by ammonium sulfate precipitation with 42.80 fold purification and specific activity of 1889.6 U/mg. Effects of various metals on enzyme activity were determined and it was found that enzyme activity boosted significantly in presence of Ca2+, K2+, Ba2+, Co2+ and Ni2+ whereas Zn2+, Mg2+, Na2+ and Cu2+ were found inhibitory at concentration 10mM.
- Subjects
AMYLASE inhibitors; BACILLUS (Bacteria); AMMONIUM sulfate; PRECIPITATION (Chemistry); ENZYME kinetics
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2016, Vol 29, Issue 4, p1275
- ISSN
1011-601X
- Publication type
Article