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- Title
The expression of acidic ribosomal phosphoproteins on the surface membrane of different tissues in autoimmune and normal mice which are the target molecules for anti-double-stranded DNA antibodies.
- Authors
Sun, K. -H.; Liu, W. -T.; Tang, S. -J.; Tsai, C. -Y.; Hsieh, S. -C.; Wu, T. -H.; Han, S. -H.; Yu, C. -L.
- Abstract
Affinity-purified polyclonal anti-double-stranded DNA (anti-dsDNA) antibodies from patients with systemic lupus erythematosus (SLE) exert a cytostatic effect on cultured rat glomerular mesangial cells (MC). The cognate antigens expressed on the surface of MC have been proved to be acidic ribosomal phosphoproteins (P proteins) in our previous study. The mesangial cytostatic effect of anti-dsDNA antibodies is attributed to the cross-reactivity of the antibodies with membrane-expressed P proteins, but not to the effect of minute amounts of anti-ribosomal P proteins antibodies contained in the anti-dsDNA preparations. Immunofluorescence staining of the native cells demonstrated that anti-dsDNA antibodies bound to the surface of rat mesangial cells, rat brain astrocytes (RBA-1) and mouse fibroblasts (3T3). Anti-dsDNA antibodies also exert potent cytostatic effects on these cells in a dose-dependent manner.
- Subjects
PHOSPHOPROTEINS; LABORATORY mice; BIOLOGICAL membranes; DNA antibodies; IMMUNOGLOBULINS; AUTOIMMUNE diseases; IMMUNOLOGY
- Publication
Immunology, 1996, Vol 87, Issue 3, p362
- ISSN
0019-2805
- Publication type
Article