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- Title
Contact Sensitivity to Oxazolone in the Mouse VIII. DEMONSTRATION OF SEVERAL CLASSES OF ANTIBODY IN THE SERA OF CONTACT SENSITIZED AND UNIMMUNTZED MICE BY A SIMPLIFIED ANTIGLOBULIN ASSAY.
- Authors
Askenase, P.W.; Asherson, G.L.
- Abstract
CBA mouse antibodies to oxazolone were assayed by direct and antiglobulin augmented hemagglutination, using oxazolone coupled sheep erythrocytes. The antiglobulin Combs' test was simplified by eliminating washing the sensitized cells and running tile entire assay in a microtitre plate. Oxazolone antibodies of IgM, IgG1 and IgG2a classes were found in unimmunized mice, and antibodies of significantly higher titre were found in IgM, IgG1, IgG2a and IgG2b classes of contact sensitized mice. Because the demonstration of oxazolone antibodies in the sera of unimmunized mice required highly oxazolonated SRBC, it was suggested that these antibodies are of relatively low binding avidity.
- Subjects
IMMUNOGLOBULINS; LABORATORY mice; OXAZOLES; BLOOD agglutination; SERUM; CONTACT dermatitis
- Publication
Immunology, 1972, Vol 23, Issue 3, p289
- ISSN
0019-2805
- Publication type
Article