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- Title
Communication between subunits within an archaeal clamp-loader complex.
- Authors
Seybert, Anja; Singleton, Martin R.; Cook, Nicola; Hall, David R.; Wigley, Dale B.
- Abstract
We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.
- Subjects
CHROMOSOME replication; ARGININE; GENETIC mutation; NUCLEOTIDES; MOLECULAR rotation; GENETICS
- Publication
EMBO Journal, 2006, Vol 25, Issue 10, p2209
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1038/sj.emboj.7601093