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- Title
Nucleosome positioning at the replication fork.
- Authors
Lucchini, Renzo; Wellinger, Ralf Erik; Sogo, José M.
- Abstract
In order to determine the time required for nucleosomes assembled on the daughter strands of replication forks to assume favoured positions with respect to DNA sequence, psoralen cross‐linked replication intermediates purified from preparative two‐dimensional agarose gels were analysed by exonuclease digestion or primer extension. Analysis of sites of psoralen intercalation revealed that nucleosomes in the yeast Saccharomyces cerevisiae rDNA intergenic spacer are positioned shortly after passage of the replication machinery. Therefore, both the 'old' randomly segregated nucleosomes as well as the 'new' assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands, suggesting that the positioning of nucleosomes is an initial step in the chromatin maturation process.
- Subjects
REPLICATION fork; NUCLEOTIDE sequence; HISTONES; CHROMATIN; SACCHAROMYCES cerevisiae; DNA sequencing; EXONUCLEASES; PSORALENS
- Publication
EMBO Journal, 2001, Vol 20, Issue 24, p7294
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/20.24.7294