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- Title
Direct observation of mammalian cell growth and size regulation.
- Authors
Son, Sungmin; Tzur, Amit; Weng, Yaochung; Jorgensen, Paul; Kim, Jisoo; Kirschner, Marc W; Manalis, Scott R
- Abstract
We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
- Subjects
REGULATION of cell growth; MICROFLUIDIC devices; CELL cycle; CELL lines; HOMEOSTASIS; PHASE transitions; MAMMALS
- Publication
Nature Methods, 2012, Vol 9, Issue 9, p910
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2133