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- Title
Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.
- Authors
Smyshlyaev, Georgy; Bateman, Alex; Barabas, Orsolya
- Abstract
Mobile genetic elements (MGEs) sequester and mobilize antibiotic resistance genes across bacterial genomes. Efficient and reliable identification of such elements is necessary to follow resistance spreading. However, automated tools for MGE identification are missing. Tyrosine recombinase (YR) proteins drive MGE mobilization and could provide markers for MGE detection, but they constitute a diverse family also involved in housekeeping functions. Here, we conducted a comprehensive survey of YRs from bacterial, archaeal, and phage genomes and developed a sequence-based classification system that dissects the characteristics of MGEborne YRs. We revealed that MGE-related YRs evolved from nonmobile YRs by acquisition of a regulatory arm-binding domain that is essential for their mobility function. Based on these results, we further identified numerous unknown MGEs. This work provides a resource for comparative analysis and functional annotation of YRs and aids the development of computational tools for MGE annotation. Additionally, we reveal how YRs adapted to drive gene transfer across species and provide a tool to better characterize antibiotic resistance dissemination.
- Subjects
PROKARYOTIC genomes; MOBILE genetic elements; RECOMBINASES; BACTERIAL genomes; SEQUENCE analysis; TYROSINE; HORIZONTAL gene transfer
- Publication
Molecular Systems Biology, 2021, Vol 17, Issue 5, p1
- ISSN
1744-4292
- Publication type
Article
- DOI
10.15252/msb.20209880