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- Title
Hydrogen Gas Enhanced Seed Germination by Increasing Trehalose Biosynthesis in Cucumber.
- Authors
Huang, Panpan; Li, Changxia; Che, Pengpeng; Liu, Huwei; Zhao, Zongxi; Feng, Li; Liu, Xinjuan; Liao, Weibiao
- Abstract
Hydrogen gas (H2) and trehalose (Tre) are involved in various biological processes in plants. In the study, the roles of H2 and Tre and their interaction during seed germination in cucumber (Cucumis sativus L.) were investigated. Our results showed that the effects of Tre on seed germination were dose-dependent, with a maximal biological response at 0.8% (w/v). The positive roles of hydrogen rich water (HRW, a H2 donor) were blocked by a specific Tre inhibitor trehazolin (Thz), suggesting that Tre might be responsible for H2-induced seed germination. HRW and Tre increased the endogenous Tre level and the activity of trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Simultaneously, the expression level of Tre metabolism-related genes was up-regulated by HRW and Tre. Additionally, the content of Tre, water soluble carbohydrate, glucose (Glu) and sucrose was increased by HRW and Tre. Meanwhile, the activity of starch-related enzymes including α-amylase (AMY), β-amylase (BMY) and total amylase enzyme was increased by HRW and Tre. Further, HRW and Tre significantly up-regulated the expression level of starch-related genes including AMY and BMY, which were inhibited by Thz, indicating that Tre might play a crucial role in H2-induced seed germination. Altogether, this study suggested that H2 might induce seed germination by increasing Tre biosynthesis in cucumber.
- Subjects
HUMAN Rights Watch (Organization); GERMINATION; TREHALOSE; CUCUMBERS; BIOSYNTHESIS; GENE expression; HYDROGEN; AMYLASES
- Publication
Journal of Plant Growth Regulation, 2023, Vol 42, Issue 6, p3908
- ISSN
0721-7595
- Publication type
Article
- DOI
10.1007/s00344-022-10858-0