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- Title
Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai.
- Authors
Yu, Chunlin; Li, Rongfeng; Yin, Xiujing; Yu, Huahua; Li, Pengcheng
- Abstract
Scyphozoan Nemopilema nomurai envenomation is an unresolved threat to human health in Asian waters. Nemopilema nomurai venom metalloproteinases show important toxicities in skin damage and inflammation, but there is still no purified protein for further studies. In this study, high proteinase activity fractions in tentacle autolysis were isolated by ammonium sulfate precipitation, DEAE Sepharose Fast Flow, and Superdex 75 chromatography successively. Purification was guided by azocasein hydrolysis activity and SDS-PAGE. The final products were analyzed by LC-MS/MS. Four elution peaks purified by Superdex 75 chromatography had multiple protein bands but did not show proteinase activity. These fractions would recover proteinase activity after mixing again. Regulation mechanisms were speculated as binding metalloproteinase regulator or disaggregating metalloproteinase inhibitor by LC-MS/MS analysis. For the first time, a synergistic effect in N. nomurai proteinase activity was found in the purification process.
- Subjects
PROTEINASES; LIQUID chromatography-mass spectrometry; AMMONIUM sulfate; SKIN inflammation; METALLOPROTEINASES; SNAKE venom; SEPHAROSE; PROTEOLYTIC enzymes
- Publication
Frontiers in Pharmacology, 2021, Vol 12, p1
- ISSN
1663-9812
- Publication type
Article
- DOI
10.3389/fphar.2021.791847