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- Title
Kupffer cell function during the erythocytic stage of malaria.
- Authors
Murthi, Padma; Kalionis, Bill; Ghabrial, Hany; Dunlop, Marjorie E.; Smallwood, Richard A.; Sewell, Richard B.
- Abstract
Background and Aim: Previous studies using isolated perfused rat liver in vivo have suggested that during the erythrocytic phase of malaria infection, overall phagocytosis by Kupffer cells is enhanced. The aim of the present study was to further investigate the individual phagocytic capacity and prostaglandin E2 (PGE2) secretion of isolated Kupffer cells in vitro, and the immunohistochemical characteristics of Kupffer cells in vivo. Methods: Malaria was induced in male Sprague–Dawley rats ( n = 12) by inoculation with parasitized red cells containing Plasmodium berghei. Kupffer cells were isolated by centrifugal elutriation. Results: A significantly increased yield of Kupffer cells was obtained from malaria-infected livers compared to controls (36.7 ± 4.5 vs 11.8 ± 1.1 ×106 cells, P < 0.0001, n = 12). There was an increased internalization by phagocytosis of [3H]-BSA latex microspheres after 60 min in malaria-infected Kupffer cells compared to controls (65.05 ± 1.5 vs 48.6 ± 0.7, P < 0.001, n = 12). PGE2 secretion into the cell culture medium was significantly suppressed in malaria-infected Kupffer cells compared to controls (1167 ± 88 vs 4537 ± 383 pg per 106 cells, P < 0.001, n = 5). Staining of ED1, ED2 and PCNA was greater in malaria-infected livers compared to control. Conclusion: The results indicate that the number of Kupffer cells is significantly increased and their phagocytic activity on a cell-by-cell basis is enhanced during the erythrocytic stage of malaria.
- Subjects
MALARIA; LABORATORY rats; KUPFFER cells; CELL physiology; PHAGOCYTOSIS; IMMUNE response; IMMUNOHISTOCHEMISTRY
- Publication
Journal of Gastroenterology & Hepatology, 2006, Vol 21, Issue 1, p313
- ISSN
0815-9319
- Publication type
Article
- DOI
10.1111/j.1440-1746.2006.04192.x