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- Title
Ultralow Laser Power Three-Dimensional Superresolution Microscopy Based on Digitally Enhanced STED.
- Authors
Shen, Xiaochun; Wang, Luwei; Li, Wei; Wang, He; Zhou, Hanqiu; Zhu, Yinru; Yan, Wei; Qu, Junle
- Abstract
The resolution of optical microscopes is limited by the optical diffraction limit; in particular, the axial resolution is much lower than the lateral resolution, which hinders the clear distinction of the three-dimensional (3D) structure of cells. Although stimulated emission depletion (STED) superresolution microscopy can break through the optical diffraction limit to achieve 3D superresolution imaging, traditional 3D STED requires high depletion laser power to acquire high-resolution images, which can cause irreversible light damage to biological samples and probes. Therefore, we developed an ultralow laser power 3D STED superresolution imaging method. On the basis of this method, we obtained lateral and axial resolutions of 71 nm and 144 nm, respectively, in fixed cells with 0.65 mW depletion laser power. This method will have broad application prospects in 3D superresolution imaging of living cells.
- Subjects
MICROSCOPY; OPTICAL limiting; HIGH resolution imaging; HIGH power lasers; THREE-dimensional imaging; OPTICAL microscopes
- Publication
Biosensors (2079-6374), 2022, Vol 12, Issue 7, pN.PAG
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios12070539