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- Title
Alteration of Signal-Transducing Molecules and Phenotypical Characteristics in Peripheral Blood Lymphocytes from Gastric Carcinoma Patients.
- Authors
Kim, Chul Woo; Choi, Sang Hoon; Chung, Eun Joo; Lee, Min Jung; Byun, Eun Kyung; Ryu, Min Hee; Bang, Yung-Jue
- Abstract
The mechanisms underlying the impaired immune response frequently observed in cancer patients are not fully understood. Alteration of T-cell-associated signal transduction molecules has recently been implicated in immune suppression in tumor-bearing hosts. Furthermore, T cells from tumor-bearing host, irrespective of the presence of the ζ-chain, showed a lack of proliferative activity and cytotoxic function. In the present study, we investigated the expression of the ζ-chain molecule and the p56lck and p59fyn protein tyrosine kinase (PTK) levels in peripheral blood T lymphocytes (T-PBL) from patients with advanced gastric carcinomas; for this, flow cytometric analysis and immunoblotting, respectively, were used. We also compared the results of flow cytometric analysis of PBL between stomach cancer patients and normal healthy volunteers. In T-PBL from 22 tumor-bearing hosts, significantly reduced ζ-chain expression (16/22, 73%) was observed. Moreover, the expression level of p56lck in T-PBL from patients was significantly lower than that of p59fyn. Flow cytometric analysis of T-PBL indicated a markedly increased CD8+28- cell population in T-PBL from 19 tumor-bearing hosts.
- Publication
Pathobiology, 1999, Vol 67, Issue 3, p123
- ISSN
1015-2008
- Publication type
Article
- DOI
10.1159/000028061