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- Title
Localization of overexpressed c-<em>myc</em> mRNA in polycystic kidneys of the <em>cpk</em> mouse.
- Authors
Harding, Michael A.; Gattone II, Vincent H.; Grantham, Jared J.; Calvet, James P.
- Abstract
The C57BL/6J-cpk mouse has a form of autosomal-recessive polycystic kidney disease characterized by the rapid growth of large collecting duct cysts and the development of severe renal failure usually by three to four weeks of age. Previous studies had shown higher steady-state levels of proto-oncogene mRNA in these cystic kidneys. It is now shown using nuclear run-on transcription that the c-fos and c-myc proto-oncogenes are transcribed at higher rates in cystic kidneys, and thus that increased transcription, in part, may account for the increased mRNA levels, c-myc mRNA was detected by in situ hybridization in nephron antagen and elongating tubules of normal and cystic kidneys during late fetal and early neonatal kidney development. Localization of c-myc expression in the normal kidney decreased with age over the three-week postnatal period. By contrast, c-myc mRNA was found in cysts as early as three days of age, with increased levels at two and three weeks, c-myc expression was also elevated in apparently normal, non-dividing proximal tubules in three-week-old cystic animals. On the basis of these findings, we suggest that c-myc expression is linked to the proliferation of cells engaged in the primary cystogenic process, and that expression of this gene in proximal tubule cells of severely azotemic animals reflects the compensatory response of residual tubular epithelial cells to progressive renal dysfunction.
- Subjects
POLYCYSTIC kidney disease; HUMAN chromosome abnormalities; CYSTIC kidney disease; MESSENGER RNA; RENAL artery diseases; KIDNEYS; NEPHROLOGY; INTERNAL medicine
- Publication
Kidney International, 1992, Vol 41, Issue 2, p317
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1992.44