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- Title
T-cell dynamics after high-dose chemotherapy in adults: elucidation of the elusive CD8<sup>+</sup> subset reveals multiple homeostatic T-cell compartments with distinct implications for immune competence.
- Authors
Fagnoni, Francesco F; Lozza, Laura; Zibera, Carlo; Zambelli, Alberto; Ponchio, Luisa; Gibelli, Nadia; Oliviero, Barbara; Pavesi, Lorenzo; Gennari, Roberto; Vescovini, Rosanna; Sansoni, Paolo; Da Prada, Gianantonio; Robustelli Della Cuna, Gioacchino
- Abstract
Summary Recovery of total T cell numbers after in vivo T-cell depletion in humans is accompanied by complex perturbation within the CD8+ subset. We aimed to elucidate the reconstitution of CD8+ T cells by separate analysis of putative naïve CD95- CD28+ , memory CD95+ CD28+ and CD28- T cell compartments after acute maximal depletion by high-dose chemotherapy (HD-ChT) in women with high-risk breast cancer. We found that recovery of putative naïve CD8+ CD95- CD28+ and CD4+ CD95- CD28+ T cells, was compatible with a thymus-dependent regenerative pathway since their recovery was slow and time-dependent, their values were tightly related to each other, and their reconstitution patterns were inversely related to age. By analysing non-naïve T cells, a striking diversion between putative memory T cells and CD28- T cells was found. These latter increased early well beyond normal values, thus playing a pivotal role in total T-cell homeostasis, and contributed to reduce the CD4 : CD8 ratio. In contrast, putative memory T cells returned to values not significantly different from those seen in patients at diagnosis, indicating that this compartment may recover after HD-ChT. At 3–5 years after treatment, naïve T cells persisted at low levels, with expansion of CD28- T cells, suggesting that such alterations may extend further. These findings indicate that CD28- T cells were responsible for ‘blind’ T-cell homeostasis, but support the notion that memory and naïve T cells are regulated separately. Given their distinct dynamics, quantitative evaluation of T-cell pools in patients undergoing chemotherapy should take into account separate analysis of naïve, memory and CD28- T cells.
- Subjects
T cells; DRUG therapy
- Publication
Immunology, 2002, Vol 106, Issue 1, p27
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1046/j.1365-2567.2002.01400.x