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- Title
Sequence diversity of resistance gene SHMT to soybean cyst nematode among wild soybeans.
- Authors
YUAN Cui-ping; ZHAO Hong-kun; WANG Yu-min; LIU Xiao-dong; QI Guang-xun; DONG Ying-shan
- Abstract
SHMT was cloned from soybean variety Forrest and reported as an SCN resistance gene at Rhg4 locus. SHMT of 92 wild soybean accessions were sequenced and their sequence diversity was analysed. A total of 13 SNPs (7 transitions and 6 transversions) and 4 Indels (all in the noncoding region) were identified, 11 of them were parsimony informative sites. Sequence diversity analysis among 92 wild soybean genotypes showed N = 0.00061 and 0=0.001 06 for SHMT. The 17 DNA variations formed 16 haplotypes, of which 9 haplotypes existed in 2 or more wild soybeans, haplotype diversity was 0. 855. 3 neutrality tests (Tajima's D, Fu and Li's F, and Z test) were not significant for the gene in these 92 wild soybean genotypes, suggesting there was no selection pressure on it. x text for distribution of alleles in resistant and susceptible accessions indicated that SHMT had no correlation with wild soybean resistance to soybean cyst nematode.92 wild soybeans were grouped into 6 clusters using UPGMA method, some relationship was found between their origin and their genetic relationship.
- Subjects
SOYBEAN varieties; PLANT diversity; SOYBEAN cyst nematode; PLANT haplotypes; NUCLEOTIDE sequence
- Publication
Chinese Journal of Oil Crop Sciences, 2014, Vol 36, Issue 6, p755
- ISSN
1007-9084
- Publication type
Article
- DOI
10.7505/j.issn.1007-9084.2014.06.010