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- Title
Detection of Proteins Related to a Salivary Glycoprotein (EP-GP).
- Authors
SCHENKELS, Leon C. P. M.; RATHMAN, W. Maud; VEERMAN, Enno C. I.; Nieuw AMERONGEN, Arie V.
- Abstract
With a highly specific monoclonal antibody against a previously isolated and characterized human salivary 19-20-kDa glycoprotein, designated as extra-parotid glycoprotein [Rathman et al. (1989) /. Biol. Buccale 17,199-208], a common epitope was detected on proteins in several excretory human body fluids. With a quantitative ELISAthe EP-GP epitope was measured in widely different concentrations in several secretory human body fluids in the descending order of seminal plasma >> tears ≈ nasal mucus ≈ sweat >> saliva. Crossreactivity was also observed in cerumen but not in milk, cerebrospinal fluid, blood plasma and urine. The relative amount of EP-GP in the positively reacting secretions was however, in the same order in each fluid per mg of protein on an average of 1% of the total protein amount. The EP-GPepitope bearing proteins found in the various human secretions were further characterized by means of electrophoresis and immunoblotting. The molecular masses and the isoelectric points of the proteins in the different secretions display strong resemblance to values found for the salivary glycoprotein EP-GP (molecular masses 19 and 20 kDa; pi values between 4.8 and 5.4). All these findings point to the presence of proteins related to EP-GP in human secretions other than saliva.
- Publication
Biological Chemistry, 1991, Vol 372, Issue 1, p325
- ISSN
1431-6730
- Publication type
Article