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- Title
The Interaction of N-Acetylcysteine and Serum Transferrin Promotes Bacterial Biofilm Formation.
- Authors
Supeng Yin; Bei Jiang; Guangtao Huang; Yulong Zhang; Bo You; Yu Chen; Yali Gong; Jing Chen; Zhiqiang Yuan; Yan Zhao; Ming Li; Fuquan Hu; Zichen Yang; Yizhi Peng
- Abstract
Background/Aims: N-acetylcysteine (NAC) is a novel and promising agent with activity against bacterial biofilms. Human serum also inhibits biofilm formation by some bacteria. We tested whether the combination of NAC and human serum offers greater anti-biofilm activity than either agent alone. Methods: Microtiter plate assays and confocal laser scanning microscopy were used to evaluate bacterial biofilm formation in the presence of NAC and human serum. qPCR was used to examine expression of selected biofilm-associated genes. Extracellular matrix (ECM) was observed by transmission electron microscopy. The antioxidants GSH or ascorbic acid were used to replace NAC, and human transferrin, lactoferrin, or bovine serum albumin were used to replace serum proteins in biofilm formation assays. A rat central venous catheter model was developed to evaluate the effect of NAC on biofilm formation in vivo. Results: NAC and serum together increased biofilm formation by seven different bacterial strains. In Staphylococcus aureus, expression of genes for some global regulators and for genes in the ica-dependent pathway increased markedly. In Pseudomonas aeruginosa, transcription of las, the PQS quorum sensing (QS) systems, and the two-component system GacS/GacA increased significantly. ECM production by S. aureus and P. aeruginosa was also enhanced. The potentiation of biofilm formation is due mainly to interaction between NAC and transferrin. Intravenous administration of NAC increased colonization by S. aureus and P. aeruginosa on implanted catheters. Conclusions: NAC used intravenously or in the presence of blood increases bacterial biofilm formation rather than inhibits it.
- Subjects
ACETYLCYSTEINE; BLOOD serum analysis; TRANSFERRIN; BIOFILMS; EXTRACELLULAR matrix; POLYMERASE chain reaction; MOLECULAR interactions
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2018, Vol 45, Issue 4, p1399
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000487566