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- Title
Combining Passive Sampling with Recombinant Receptor-Reporter Gene Bioassays to Assess the Receptor Activity of Victorian Rivers.
- Authors
Allinson, Graeme; Shiraishi, Fujio; Kamata, Ryo; Allinson, Mayumi
- Abstract
This pilot study was initiated to provide new information on the 'hormonal' activity of Victorian rivers. Chemcatcher™ passive sampler systems containing Empore™ C18FF disks were deployed at eight riverine sites near Melbourne. Little estrogenic activity [<0.4-1.8 ng estradiol equivalents (EQ)/disk] and no retinoic acid activity (RAR, all samples <0.8 ng trans-retinoic acid EQ/disk) was observed. Almost all sample extracts showed aryl hydrocarbon receptor activity (from <4 to 29 ng β-naphthoflavone EQ/disk). Overall, the disk extracts were eminently compatible with the bioassay screening technology, enabling the relative levels of 'hormonal activity' to be observed in the surface waters in and around Melbourne. From a practical perspective, the in situ sampling and pre-concentration provided by passive sampling reduces the manual handling risks associated with sample transport, and the number of laboratory operations required to obtain assay-ready solutions for analysis.
- Subjects
AUSTRALIA; BIOLOGICAL assay; ARYL hydrocarbon receptors; RIVERS; FLAVONES; SOLUTION (Chemistry); WATER sampling
- Publication
Bulletin of Environmental Contamination & Toxicology, 2015, Vol 95, Issue 6, p758
- ISSN
0007-4861
- Publication type
Article
- DOI
10.1007/s00128-015-1577-6