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- Title
A Mini-Review of Diagnostic Methods for the Antigen and Antibody Detection of Rocky Mountain and Brazilian Spotted Fever.
- Authors
Silva, Kamila Alves; Prado, Vanesa Borges do; Silva, Rafael Rodrigues; Rocha, Marcelo van Petten; de Oliveira, Rafael Almeida Ribeiro; Falcão, Tarumim de Jesus Rodrigues; Serpa, Clara Cristina; Rocha, Marina Andrade; Pereira, Sabrina Paula; Silva, Líria Souza; Machado, Juliana Martins; Machado-de-Ávila, Ricardo Andrez; Fujiwara, Ricardo Toshio; Chávez-Fumagalli, Miguel Angel; Coelho, Eduardo Antônio Ferraz; Giunchetti, Rodolfo Cordeiro; Campos-da-Paz, Mariana; Gonçalves, Ana Alice Maia; Galdino, Alexsandro Sobreira
- Abstract
Rocky Mountain or Brazilian spotted fever, caused by Rickettsia rickettsii, is a fulminant, seasonal, and neglected disease that occurs in focal points of North America and South America. Its rapid detection is essential for the better prognosis and survival rate of infected individuals. However, disease diagnosis still faces challenges as the accuracy of many of the available laboratory tests fluctuates. This review aimed to analyze methods for antibody or antigen detection, their gaps, and their evolution over time. A search was conducted to find all studies in the Pubmed database that described the antibody or antigen detection of R. rickettsii infections. Initially, a total of 403 articles were screened. Of these articles, only 17 fulfilled the pre-established inclusion criteria and were selected. Among the different methods applied, the IFA technique was the one most frequently found in the studies. However, it presented varied results such as a low specificity when using the indirect method. Other techniques, such as ELISA and immunohistochemistry, were also found, although in smaller numbers and with their own limitations. Although some studies showed promising results, there is a pressing need to find new techniques to develop a rapid and effective diagnosis of R. rickettssi infection.
- Subjects
DIAGNOSIS; NEGLECTED diseases; SURVIVAL rate; DATABASES; RICKETTSIA
- Publication
Biomedicines, 2024, Vol 13, Issue 7, p1501
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines12071501