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- Title
In vitro endothelial hyperpermeability occurs early following traumatic hemorrhagic shock.
- Authors
van Leeuwen, Anoek L.I.; Naumann, David N.; Dekker, Nicole A.M.; Hordijk, Peter L.; Hutchings, Sam D.; Boer, Christa; van den Brom, Charissa E.
- Abstract
BACKGROUND: Endothelial hyperpermeability is suggested to play a role in the development of microcirculatory perfusion disturbances and organ failure following hemorrhagic shock, but evidence is limited. OBJECTIVE: To study the effect of plasma from traumatic hemorrhagic shock patients on in vitro endothelial barrier function. METHODS: Plasma from traumatic hemorrhagic shock patients was obtained at the emergency department (ED), the intensive care unit (ICU), 24 h after ICU admission and from controls (n = 8). Sublingual microcirculatory perfusion was measured using incident dark field videomicroscopy at matching time points. Using electric cell-substrate impedance sensing, the effects of plasma exposure on in vitro endothelial barrier function of human endothelial cells were assessed. RESULTS: Plasma from traumatic hemorrhagic shock patients collected at ED admission induced a 19% loss of in vitro endothelial resistance compared to plasma from controls (p < 0.001). This loss was due to reduced cell-cell contacts (p < 0.01). Plasma withdrawn at later time points did not affect endothelial barrier function (p > 0.99). Interestingly, in vitro endothelial resistance showed a positive association with in vivo microcirculatory perfusion (r = 0.56, p < 0.01). CONCLUSIONS: Plasma from traumatic hemorrhagic shock patients obtained following ED admission, but not at later stages, induced in vitro endothelial hyperpermeability. This coincided with in vivo microcirculatory perfusion disturbances.
- Subjects
HEMORRHAGIC shock; TRAUMATIC shock (Pathology); ELECTRIC impedance; INTENSIVE care units; PLASMA confinement
- Publication
Clinical Hemorheology & Microcirculation, 2020, Vol 75, Issue 2, p121
- ISSN
1386-0291
- Publication type
Article
- DOI
10.3233/CH-190642