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- Title
Analysis of Natural Selection of Immune Genes in Spinibarbus caldwelli by Transcriptome Sequencing.
- Authors
Tuo, Yun; Chu, Wuying; Zhang, Jianshe; Cheng, Jia; Chen, Lin; Bao, Lingsheng; Xiao, Tiaoyi
- Abstract
Spinibarbus caldwelli is an omnivorous cyprinid fish that is distributed widely in China. To investigate the adaptive evolution of S. caldwelli , the muscle transcriptome was sequenced by Illumina HiSeq 4000 platform. A total of 80,447,367 reads were generated by next-generation sequencing. Also, 211,386 unigenes were obtained by de novo assembly. Additionally, we calculated that the divergence time between S. caldwelli and Sinocyclocheilus grahami is 23.14 million years ago (Mya). And both of them diverged from Ctenopharyngodon idellus 46.95 Mya. Furthermore, 38 positive genes were identified by calculating Ka/Ks ratios from 9225 orthologs. Among them, several immune-related genes were identified as positively selected, such as POLR3B , PIK3C3 , TOPORS , FASTKD3 , CYPLP1A1 , and UACA. Our results throw light on the nature of the natural selection of S. caldwelli and contribute to future immunological and transcriptome studies.
- Subjects
CHINA; CTENOPHARYNGODON idella; GENES; NUCLEOTIDE sequencing
- Publication
Frontiers in Genetics, 2020, Vol 11, p1
- ISSN
1664-8021
- Publication type
Article
- DOI
10.3389/fgene.2020.00714