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- Title
Identification of a Protein that Interacts with LeATL6 Ubiquitin-protein Ligase E3 Upregulated in Tomato Treated with Elicitin-Like Cell Wall Proteins of Pythium oligandrum.
- Authors
Takahashi, Hideki; Hase, Shu; Kanayama, Yoshinori; Takenaka, Shigehito
- Abstract
The expression of LeATL6, which encodes RING-H2 zinc finger ubiquitin-protein ligase E3, is highly induced in tomato roots treated with the elicitin-like cell wall protein fraction (CWP) from the non-pathogenic oomycete Pythium oligandrum, which enhances resistance to pathogens through a jasmonic acid (JA)-dependent signalling pathway. In this study, the role of LeATL6 for CWP-induced defence response was further analysed. To screen the putative target protein of LeATL6 for the CWP-induced defence mechanism in tomato, we used a yeast two-hybrid system to screen five clones encoding a protein that interacts with LeATL6. Four clones had a function associated with the ubiquitin-proteasome system. Another positive clone encoded a protein sharing homology with S-adenosylmethionine decarboxylase (SAMDC). In CWP-treated tomato roots, SAMDC activity was clearly suppressed. Thus, the interaction of SAMDC with LeATL6 and the decreased SAMDC activity may be associated with JA-dependent induced resistance in tomato treated with P. oligandrum.
- Subjects
ZINC-finger proteins; UBIQUITIN; PYTHIUM; PLANT cell walls; PATHOGENIC microorganisms
- Publication
Journal of Phytopathology, 2010, Vol 158, Issue 2, p132
- ISSN
0931-1785
- Publication type
Article
- DOI
10.1111/j.1439-0434.2009.01589.x