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- Title
VARIANTS IN VDR AND NRAMP1 GENES AS SUSCEPTIBILITY FACTORS FOR TUBERCULOSIS IN THE POPULATION OF SERBIA.
- Authors
TRIFUNOVIĆ, Vesna ŠKODRIĆ; BUHA, Ivana; JOVANOVIĆ, Dragana; VUĈINIĆ, Violeta; STJEPANOVIĆ, Mihailo; SPASOVSKI, Vesna; ANDJELKOVIĆ, Marina; VREĆA, Miša; SKAKIĆ, Anita; GAŠIĆ, Vladimir; PAVLOVIĆ, Sonja
- Abstract
Tuberculosis (TB) is granulomatous diseases caused by Mycobacterium tuberculosis (MTB). TB is a highly infectious disease that primarily affects the lungs. One-third of human population is infected with MTB, therefore it is of utmost significance to determine the factors that influence the individual susceptibility to the disease. Host genetic factors have been recognized as essential for susceptibility to TB, since only 5% to 10% of infected individuals develop the disease. A number of candidate genes has been intensively studied, the most of which were connected with the function of macrophages, thus participating in immune response. Here we examined the gene variants of VDR (FokI) and NRAMP1 (INT4, D543N, 3'UTR) genes in aim to make the correlation between these genetic factors and risk of TB in Serbian patients. This study included 110 TB patients and 67 healthy controls. Pulmonary TB was diagnosed by clinical symptoms, radiological evidence of TB and bacteriological criteria (Culture- positive/ smear- positive). Genotyping was performed using PCR-RFLP method. Our findings revealed significant prevalence of ff genotype and variant allele f of the FokI VDR gene variant in patients compared to control group. Based on the our results the carriers of ff genotype are five times more at risk to tuberculosis than carriers of FF and Ff genotype in our population. The results of analyzed SNPs in NRAMP1 gene showed no statistically significant difference in distribution of the gene variants between patient and control groups. Therefore, we could conclude that the genotype ff of the VDR gene is factor that strongly contribute to susceptibility to TB in Serbian population.
- Subjects
SERBIA; TUBERCULOSIS; DISEASE susceptibility; POPULATION genetics; MACROPHAGES; STATISTICAL correlation; DISEASE prevalence; CONTROL groups
- Publication
Genetika (0534-0012), 2015, Vol 47, Issue 3, p1021
- ISSN
0534-0012
- Publication type
Article
- DOI
10.2298/GENSR1503021T