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- Title
Dissecting the genetic background of stem rust resistance present in the winter wheat cultivar Mv Zelma.
- Authors
Mayer, Marianna; Karsai, Idikó; Láng, László; Veisz, Ottó; Vida, Gyula
- Abstract
Stem rust, caused by the fungus Puccinia graminis f. sp. tritici Eriks. & E. Henn., is an impending disease for wheat production worldwide hence breeding for stem rust resistance is a very important challenge of wheat breeders all around the world. Although the disease has been under effective control in Hungary through the use of host resistance, there are needs for new sources of resistance because of the threatening new pathogen races in Africa and Middle East. The Hungarian winter wheat variety ʽMv Zelma' possesses a high level of resistance against the local stem rust races being durable over the past years and in addition, this variety proved to be resistant against the Ug99 race-group, as well. The main objective of this work was to identify the genetics of resistance in Mv Zelma. We crossed Mv Zelma with two susceptible Mv lines, Mv12-13 and Mv32-13. We started the anther culture from the anthers of the F2 plants, in order to fix the F3 segregation in homozygous state by creating doubled haploid lines. We investigated the frequency of the Sr38 and Sr15 stem rust resistance genes in both populations and the parents using the gene specific VENTRIUP-LN2 (for Sr38) and STS638 (for Sr15) molecular markers (Helguera et al. 2003), (Neu et al. 2002). The stem rust resistance of the plants were scored in adult plant stage by the modified Cobb scale, evaluating the reaction type and the severity at the same time. The field experiments were set up in two replications in the artificially inoculated stem rust nursery of the Agricultural Institute, Centre for Agricultural Research, Martonvásár in 2017. Statistical analysis of the data was performed to determine the connection between the two Sr genes and the phenotypic responses of the doubled haploid lines. Both of the stem rust resistance genes originated from Mv Zelma. Based on the phenotypic data, the Sr38 resistance gene showed a very strong correlation with the resistance level of the doubled haploid lines. This major gene alone explained 75.6% and 74.9% of the phenotypic variation in stem rust resistance and its presence reduced the Cobb scale values in the Mv12-13/Mv Zelma and Mv32-13/Mv Zelma doubled haploid lines with values of 71.6 and 68.5, respectively. On the other hand, Sr15 gene alone had no significant effect on the susceptibility of the doubled haploid lines. We identified however a cross specific interaction between Sr38 and Sr15. In the case of the Mv12-13/Mv Zelma lines, Sr15 seemed to decrease the protecting effect of the Sr38, while in the Mv32-13/Mv Zelma combination Sr15 had no significant effect on Sr38. The results prove that the resistance of Mv Zelma against the Hungarian races of Puccinia graminis f.sp. tritici is primarily due to the presence of the Sr38 resistance gene.
- Subjects
WHEAT disease &; pest resistance; PUCCINIA graminis; WINTER wheat
- Publication
Cereal Research Communications, 2017, Vol 45, p86
- ISSN
0133-3720
- Publication type
Abstract
- DOI
10.1556/0806.45.2017.100