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- Title
Moesin (MSN) as a Novel Proteome-Based Diagnostic Marker for Early Detection of Invasive Bladder Urothelial Carcinoma in Liquid-Based Cytology.
- Authors
Park, Jeong Hwan; Lee, Cheol; Han, Dohyun; Lee, Jae Seok; Lee, Kyung Min; Song, Min Ji; Kim, Kwangsoo; Lee, Heonyi; Moon, Kyung Chul; Kim, Youngsoo; Jung, Minsun; Moon, Ji Hye; Lee, Hyebin; Ryu, Han Suk
- Abstract
Bladder urothelial carcinoma (BUC) is the most lethal malignancy of the urinary tract. Treatment for the disease highly depends on the invasiveness of cancer cells. Therefore, a predictive biomarker needs to be identified for invasive BUC. In this study, we employed proteomics methods on urine liquid-based cytology (LBC) samples and a BUC cell line library to determine a novel predictive biomarker for invasive BUC. Furthermore, an in vitro three-dimensional (3D) invasion study for biological significance and diagnostic validation through immunocytochemistry (ICC) were also performed. The proteomic analysis suggested moesin (MSN) as a potential biomarker to predict the invasiveness of BUC. The in vitro 3D invasion study showed that inhibition of MSN significantly decreased invasiveness in BUC cell lines. Further validation using ICC ultimately confirmed moesin (MSN) as a potential biomarker to predict the invasiveness of BUC (p = 0.023). In conclusion, we suggest moesin as a potential diagnostic marker for early detection of BUC with invasion in LBC and as a potential therapeutic target.
- Subjects
BLADDER tumors; BODY fluid examination; CANCER invasiveness; CYTODIAGNOSIS; IMMUNOHISTOCHEMISTRY; MEMBRANE proteins; TUMOR markers; URINALYSIS; PROTEOMICS; EARLY detection of cancer; IN vitro studies
- Publication
Cancers, 2020, Vol 12, Issue 4, p1018
- ISSN
2072-6694
- Publication type
Article
- DOI
10.3390/cancers12041018