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- Title
Pikromycin production stimulation through antibiotic down-regulatory gene disruption in Streptomyces venezuelae.
- Authors
Woo, Min-Woo; Nah, Hee-Ju; Choi, Si-Sun; Kim, Eung-Soo
- Abstract
The wblA gene, which encodes a whiB-like putative transcription factor, has been widely reported as an antibiotic biosynthesis down-regulator in Streptomyces coelicolor. The wblA ortholog from Streptomyces venezuelae was identified by sequence alignment with wblA from S. coelicolor. To determine the biological significance of wblA from S. venezuelae, chromosomal disruption of the wblA gene was performed by homologous recombination using the pKC1139 vector. The phenotypic difference between S. venezuelae and S. venezuelaeΔwblA was shown, and pikromycin production was quantified by HPLC analysis. Production of pikromycin by S. venezuelaeΔwblA was approximately 3.5-fold higher compared to that by S. venezuelae. To further show that the wblA gene was implicated in pikromycin production, wblA was cloned into Streptomyces integrative expression vector, which was independently introduced into both S. venezuelae and S. venezuelaeΔwblA by conjugation. HPLC analysis of complementation and overexpression strains showed an approximately 2.5-fold reduction in pikromycin production by the wblA overexpression mutant.
- Subjects
STREPTOMYCES venezuelae; ANTIBIOTIC synthesis; ENDOPEPTIDASES; ENZYME inhibitors; DOWNREGULATION; TRANSCRIPTION factors; GENETIC overexpression; DNA damage
- Publication
Biotechnology & Bioprocess Engineering, 2014, Vol 19, Issue 6, p973
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-014-0407-8