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- Title
Rapid identification of dengue virus by reverse transcription-polymerase chain reaction using field-deployable instrumentation.
- Authors
McAvin JC; Escamilla EM; Blow JA; Turell MJ; Quintana M; Bowles DE; Swaby JA; Barnes WJ; Huff WB; Lohman KL; Atchley DH; Hickman JR; Niemeyer DM; McAvin, James C; Escamilla, Elizabeth M; Blow, Jamie A; Turell, Michael J; Quintana, Miguel; Bowles, David E; Swaby, James A
- Abstract
Dengue virus universal and dengue serotype 1 to 4, fluorogenic probe hydrolysis (TaqMan), reverse transcription-polymerase chain reaction assays were developed for screening and serotype identification of infected mosquito vectors and human sera using a field-deployable, fluorometric thermocycler. Dengue universal and dengue 1 to 4 serotype assay in vitro sensitivity and specificity results were 100% concordant when tested with total nucleic acid extracts of multiple strains of dengue serotype 1 to 4, yellow fever, Japanese encephalitis, West Nile, and St. Louis encephalitis viruses. The in vitro sensitivity and specificity results for all five assays were concordant when tested with a blind panel of 27 dengue virus-infected mosquitoes, 21 non-dengue (yellow fever, West Nile, or St. Louis encephalitis) flavivirus-infected mosquitoes, and 11 uninfected mosquitoes and with clinical specimens consisting of a human serum panel of eight dengue viremic and 31 non-dengue-infected febrile patient serum samples. No cross-reaction occurred with vector species or human genomic DNA. Sample processing and polymerase chain reaction required <2 hours.
- Publication
Military Medicine, 2005, Vol 170, Issue 12, p1053
- ISSN
0026-4075
- Publication type
journal article
- DOI
10.7205/milmed.170.12.1053