We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Validation of CSF-1 receptor (CD115) staining for analysis of murine monocytes by flow cytometry.
- Authors
Kapanadze, Tamar; Gamrekelashvili, Jaba; Sablotny, Stefan; Schroth, Frauline Nicole; Xu, Yuangao; Chen, Rongjun; Rong, Song; Shushakova, Nelli; Gueler, Faikah; Haller, Hermann; Limbourg, Florian P
- Abstract
CD115, the receptor for colony stimulating factor 1, is essential for survival and differentiation of monocytes and macrophages and is therefore frequently used to define monocyte subsets and their progenitors in immunological assays. However, CD115 surface expression and detection by flow cytometry is greatly influenced by cell isolation and processing methods, organ source, and disease context. In a systematic analysis of murine monocytes, we define experimental conditions that preserve or limit CD115 surface expression and staining by flow cytometry. We also find that, independent of conditions, CD115 surface levels are consistently lower in Ly6Clo monocytes than in Ly6Chi monocytes, with the exception of Ly6Clo monocytes in the bone marrow. Furthermore, in contrast to IL-34, the presence of colony stimulating factor 1 impairs CD115 antibody staining in a dose-dependent manner, which, in a model of ischemic kidney injury with elevated levels of colony stimulating factor 1, influenced quantification of kidney monocytes. Thus, staining and experimental conditions affect quantitative and qualitative analysis of monocytes and may influence experimental conclusions.
- Subjects
MACROPHAGE colony-stimulating factor; FLOW cytometry; MONOCYTES; BONE marrow; CELL separation
- Publication
Journal of Leukocyte Biology, 2024, Vol 115, Issue 3, p573
- ISSN
0741-5400
- Publication type
Article
- DOI
10.1093/jleuko/qiad147