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- Title
The effect of nicotine on the production of soluble fms-like tyrosine kinase-1 and soluble endoglin in human umbilical vein endothelial cells and trophoblasts.
- Authors
JA-YOUNG KWON; SANG-WOOK BAI; YOUNG-GUEN KWON; SE-HOON KIM; CHUL HOON KIM; MOUNG HWA KANG; LINTON, JOHN A.; YONG-WON PARK; Kwon, Ja-Young; Bai, Sang-Wook; Kwon, Young-Guen; Kim, Se-Hoon; Kim, Chul Hoon; Kang, Moung Hwa; Park, Yong-Won
- Abstract
<bold>Objectives: </bold>To evaluate the effect of nicotine on the production of soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) in human umbilical vein endothelial cells (HUVECs) and trophoblast cells, and to assess the involvement of alpha 7 nicotinic acetylcholine receptor (alpha7 nAChR) in this process.<bold>Methods: </bold>Commercially available full-term placental trophoblasts and HUVECs derived from the umbilical cord of a normal pregnancy were used. The expression of alpha7 nAChR was assessed by immunostaining, RT-PCR, and western blotting. The expression of sFlt-1 and sEng protein in the cell media after 6 and 24 hours of treatment with nicotine was evaluated using a commercially available ELISA. To determine the involvement of alpha7 nAChR in the nicotinic effect, cells were treated with the alpha7 nAChR antagonist alpha-bungarotoxin (alpha-BGT) prior to the nicotine exposure. Levels of significance were determined using the Student's t-test and one-way ANOVA, and a p-value < 0.05 was considered significant.<bold>Main Outcome Measures: </bold>The levels of sFlt-1 and sEng protein were evaluated before and after the nicotine treatment with or without alpha-BGT pre-treatment.<bold>Results: </bold>In trophoblast cells, a significant reduction of sFlt-1 and sEng protein was observed after 24 hours of nicotine treatment as compared to the untreated group (p = 0.002, 0.000). In HUVECs, nicotine only had a suppressive effect on the expression of sEng at 6 hours (p = 0.03); there was no effect on sFlt-1 expression. However, pre-treatment with alpha-BGT did not reverse the nicotine-induced suppressive effect on the expression of sFlt-1 and sEng in trophoblasts and HUVECs.<bold>Conclusions: </bold>Nicotine reduced the production of sFlt-1 and sEng in trophoblasts and sEng in HUVECs. This effect was not mediated by alpha7 nAChR.
- Subjects
NICOTINE; PROTEIN-tyrosine kinases; ENDOTHELIUM; TROPHOBLAST; BLASTOCYST
- Publication
Acta Obstetricia et Gynecologica Scandinavica, 2010, Vol 89, Issue 4, p565
- ISSN
0001-6349
- Publication type
journal article
- DOI
10.3109/00016341003692253