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- Title
Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from Arthrobacter sp. CSAJ-16 in Cangshan Mountain.
- Authors
Yan, Xin; Hu, Wei; Zhu, Yun-Guo; Liu, Qing-Qing; Wang, Shuai; Liu, Hong-Yan; Zhu, Dan; Lv, Zhi-Hua; Li, Lin-Hua; Yin, Yi-Rui
- Abstract
Uricase (or Urate oxidase), a key enzyme involved in purine metabolism, is commonly used in treating conditions such as gout, hyperuricemia, and tumor lysis syndrome. In this study, a uricase-producing strain (named CSAJ-16) was isolated from the soil sample of Cangshan Mountain, Yunnan Province, China. This strain was identified as Arthrobacter sp. CSAJ-16. Based on the gene sequence alignment, the uricase gene (named aruox) of Arthrobacter sp. CSAJ-16 was amplified and heterologously expressed. The recombinant uricase (ArUOX) was about 32 kDa. The optimal pH and temperature of ArUOX were pH 7 and 20°C, respectively. The ArUOX remained above 50% relative activity after incubation at 37°C for 100 min or at pH 6.0–8.6 for 24 h. Moreover, metal ions such as K+, Mg2+, Ca2+, Ba2+ and Pb2+ can significantly enhance the activity of ArUOX (> 200%). These enzymatic properties indicate that ArUOX has potential applications in pharmaceutical enzymes and uric acid detection kits.
- Subjects
YUNNAN Sheng (China); MOLECULAR cloning; ARTHROBACTER; TUMOR lysis syndrome; SEQUENCE alignment; URIC acid
- Publication
Polish Journal of Microbiology, 2023, Vol 72, Issue 3, p277
- ISSN
1733-1331
- Publication type
Article
- DOI
10.33073/pjm-2023-027