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- Title
Determination of carbapenems in water samples by UHPLC–MS/MS.
- Authors
Wang, Chengfei; Li, Xiaowei; Lv, Ziquan; Wang, Yingyu; Ke, Yuebin; Xia, Xi
- Abstract
A rapid and reliable method for the detection of five carbapenems (biapenem, imipenem, doripenem, meropenem, and faropenem) in water was developed and validated. After acidification of water samples with acetic acid, carbapenems were isolated using a Bond Elut PPL cartridge. The target compounds were separated using ultra high performance liquid chromatography with a chromatographic run time of 5 min and detected on a triple quadrupole mass spectrometer operated in positive electrospray ionization and multiple reaction monitoring mode. Mean recoveries were in the range of 76.6–106.5%, with satisfactory intraday and interday relative standard deviations lower than 10.0 and 10.8%, respectively. The limits of detection and quantification were in the ranges of 0.05–0.2 µg/L and 0.1–0.5 µg/L, respectively, depending on the analyte. The proposed method was applied to the analysis of river samples and wastewater samples from swine farms, and no carbapenems were detected in the collected samples.
- Subjects
CARBAPENEMS; HIGH performance liquid chromatography; WATER sampling; SWINE farms; TANDEM mass spectrometry; WATER acidification; MASS spectrometers
- Publication
Journal of Separation Science, 2020, Vol 43, Issue 12, p2321
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.201901343