We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Human BIN1 isoforms grow, maintain, and regenerate excitation–contraction couplons in adult rat and human stem cell-derived cardiomyocytes.
- Authors
Guo, Jia; Tian, Qinghai; Barth, Monika; Xian, Wenying; Ruppenthal, Sandra; Schaefers, Hans-Joachim; Chen, Zhifen; Moretti, Alessandra; Laugwitz, Karl-Ludwig; Lipp, Peter
- Abstract
Aims In ventricular myocytes, transverse-tubules (T-tubules) are instrumental for excitation–contraction (EC)coupling and their disarray is a hallmark of cardiac diseases. BIN1 is a key contributor to their biogenesis. Our study set out to investigate the role of human BIN1 splice variants in the maintenance and regeneration of EC-coupling in rat adult ventricular myocytes and human-induced pluripotent stem cell-derived cardiac myocytes (hiPS-CMs). Methods and results In heart samples from healthy human donors expression patterns of five BIN1 splice variants were identified. Following viral transduction of human BIN1 splice variants in cellular models of T-tubular disarray, we employed high-speed confocal calcium imaging and CaCLEAN analysis to identify functional EC-coupling sites (couplons) and T-tubular architecture. Adult rat ventricular myocytes were used to investigate the regeneration after loss and maintenance of EC-coupling while we studied the enhancement of EC-coupling in hiPS-CMs. All five human BIN1 splice variants induced de-novo generation of T-tubules in both cell types. Isoforms with the phosphoinositide-binding motif (PI) were most potent in maintenance and regeneration of T-tubules and functional EC-coupling in adult rat myocytes. In hiPSC-CMs, BIN1 variants with PI-motif-induced de novo generation of T-tubules, functional couplons and enhanced calcium handling. Conclusion BIN1 is essential for the maintenance, regeneration, and de novo generation of functional T-tubules. Isoforms with PI-motifs appeared as particulalrly potent. These T-tubules trigger the development of functional couplons resulting in enhanced calcium handling.
- Subjects
CARDIAC regeneration; RATS; HEART diseases; MUSCLE cells; ADULTS; PHOSPHOINOSITIDES
- Publication
Cardiovascular Research, 2022, Vol 118, Issue 6, p1479
- ISSN
0008-6363
- Publication type
Article
- DOI
10.1093/cvr/cvab195