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- Title
Expression patterns of miR-221 and its target Caspase-3 in different cancer cell lines.
- Authors
Ergun, Sercan; Arman, Kaifee; Temiz, Ebru; Bozgeyik, İbrahim; Yumrutaş, Önder; Safdar, Muhammad; Dağlı, Hasan; Arslan, Ahmet; Oztuzcu, Serdar
- Abstract
Caspases are important initiators and most well-known finishers of apoptosis. By changing the death propagation homeostatic equilibrium, their different expression patterns might trigger the progression of hazardous diseases like cancer. miR-221 is an oncogenic miRNA. It is known to have both anti-angiogenic and angiogenic effect. The aim of this work was to compare the expression levels of miR-221 and its target caspase-3 in different cancer cell lines and to find out a relationship between these two. We also tried to establish a prominent relationship between miR-221 and its role in apoptosis by studying their expression levels. Our results indicate that expression of caspase-3 is quite lower as compared to miR-221 expression in all of the selected cancer cell lines. As a result, we conclude that miR-221 may have a crucial role in repressing the expression of caspase-3 which may contribute to a lower apoptotic rate, thus supporting the selection of more aggressive cancer cells. To our knowledge, this is the first study related to the expression levels of caspase-3 and miR-221 in different cell lines at the same time. We expect that our study might pave the way for better understanding the role of miR-221 in apoptotic regulation of caspase-3.
- Subjects
APOPTOSIS; CASPASE genetics; CANCER cells; CELL lines; GENE expression; MICRORNA; GENETICS
- Publication
Molecular Biology Reports, 2014, Vol 41, Issue 9, p5877
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-014-3461-6