We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The Role Of P115 Rhogef In The Rho Mediated Effects Of Lpa On The Actin Cytoskeleton.
- Authors
Bailey, GW; Jerome, WG; McKernan, S; Mansfield, JF; Price, RL; Schwartz, CL; Wells, C; Jiang, X; Arnott, HJ; Sternweis, PC; Wilk-Blaszczak, M A
- Abstract
In the fibroblast cell line, 3T3, lysophosphatidic acid (LPA) induces stress fiber formation. Stress fibers participate in physiological functions such as cell motility. LPA acts through a receptor coupled to a PTX-insensitive G-protein, G13. It was shown that a constitutively activated mutant of α13 (Q226L) induces stress fiber formation in Swiss3T3 cells through a second messenger cascade that involves a monomeric G-protein, Rho. The recently discovered guanine nucleotide exchange factor, p115 RhoGEF (p115) forms a link between a n and Rho A. In the presence of α13, p115 activates Rho. The N-terminus of p115 contains a regulator of G-protein signaling (RGS) box. RGS proteins act as negative regulators of G-protein dependent signaling by increasing GTPase activity and "locking" the G-protein in an inactive state. We have tested a role of p115 in the pathway coupling the LPA receptor to stress fiber formation by Rho in NTH-3T3 cells.
- Publication
Microscopy & Microanalysis, 1999, p1326
- ISSN
1431-9276
- Publication type
Article
- DOI
10.1017/S1431927600019954