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- Title
Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.
- Authors
Wosen, Jonathan E.; Ilstad-Minnihan, Alexandra; Co, Julia Y.; Jiang, Wei; Mukhopadhyay, Dhriti; Fernandez-Becker, Nielsen Q.; Kuo, Calvin J.; Amieva, Manuel R.; Mellins, Elizabeth D.
- Abstract
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.
- Subjects
EPITHELIUM; EPITHELIAL cells; STEM cells; IN vitro studies; ANTIGEN presentation
- Publication
Frontiers in Immunology, 2019, p1
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2019.01970